Abstract
Lentinula edodes, one of the most popular, edible mushroom species with a high content of proteins and polysaccharides as well as unique aroma, is widely cultivated in many Asian countries, especially in China, Japan and Korea. As a white rot fungus with lignocellulose degradation ability, L. edodes has the potential for application in the utilization of agriculture straw resources. Here, we report its 41.8-Mb genome, encoding 14,889 predicted genes. Through a phylogenetic analysis with model species of fungi, the evolutionary divergence time of L. edodes and Gymnopus luxurians was estimated to be 39 MYA. The carbohydrate-active enzyme genes in L. edodes were compared with those of the other 25 fungal species, and 101 lignocellulolytic enzymes were identified in L. edodes, similar to other white rot fungi. Transcriptome analysis showed that the expression of genes encoding two cellulases and 16 transcription factor was up-regulated when mycelia were cultivated for 120 minutes in cellulose medium versus glucose medium. Our results will foster a better understanding of the molecular mechanism of lignocellulose degradation and provide the basis for partial replacement of wood sawdust with agricultural wastes in L. edodes cultivation.
Highlights
Lentinula edodes, known as Xianggu or shiitake, belonging to the Agaricales order of the Agaricomycetes class in Basidiomycota phylum, is one of the white-rot fungi that grow on the dead tree or sawdust by degrading cellulose, hemicellulose and lignin
We report a draft genome sequence of monokaryotic L. edodes strain W1-26, and the identification of a large set of genes and potential gene clusters involved in lignocellulose degradation
84% of the genes were annotated in similarity searches against homologous sequences and protein domains (S5 Table, S2 Fig and S3 Fig)
Summary
Known as Xianggu or shiitake, belonging to the Agaricales order of the Agaricomycetes class in Basidiomycota phylum, is one of the white-rot fungi that grow on the dead tree or sawdust by degrading cellulose, hemicellulose and lignin. L. edodes is the second most widely cultivated mushroom species all over the world, only second to Agaricus bisporus [1,2,3]. As a delicious edible mushroom initially cultivated more than eight.
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