Abstract
Segmented filamentous bacteria (SFB) are well appreciated for eliciting Th17 cell immune responses. Here, we report the genome sequence of a murine isolate of SFB, which confers strong protection against rotavirus infection independent of acquired immunity.
Highlights
Segmented filamentous bacteria (SFB), are spore-forming bacteria that belong to the phylum of Firmicutes, in the order of Clostridiales [1, 2]
The total numbers of raw reads generated for SFB-P and SFB-G are 19.6 million and 21.89 million, respectively
We collected the so-called SFB reads by mapping reads to all 13 SFB genome sequences deposited in NCBI and extracted all the reads that aligned to any of the SFB genomes via BWA aligners
Summary
Segmented filamentous bacteria (SFB), are spore-forming bacteria that belong to the phylum of Firmicutes, in the order of Clostridiales [1, 2]. The SFB-P strain was maintained by monoassociation of GF mice [5]. DNA was extracted from feces and cecal contents of the SFB-mono-associated mice and sequenced on an Illumina HiSeq 2500 instrument, which generates paired 125-bp reads, using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA). Sequences from 2 cecum samples, harvested from 2 littermate mice, and 1 fecal sample were combined for the assembly of SFB-G.
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