Abstract
Candida oleophila is an effective biocontrol agent used to control post-harvest diseases of fruits and vegetables. C. oleophila I-182 was the active agent used in the first-generation yeast-based commercial product, Aspire®, for post-harvest disease management. Several action modes, like competition for nutrients and space, induction of pathogenesis-related genes in host tissues, and production of extracellular lytic enzymes, have been demonstrated for the biological control activity exhibited by C. oleophila through which it inhibits post-harvest pathogens. In the present study, the whole genome of C. oleophila I-182 was sequenced using PacBio and Illumina shotgun sequencing technologies, yielding an estimated genome size of 14.73 Mb. The genome size is similar in length to that of the model yeast strain Saccharomyces cerevisiae S288c. Based on the assembled genome, protein-coding sequences were identified and annotated. The predicted genes were further assigned with gene ontology terms and clustered in special functional groups. A comparative analysis of C. oleophila proteome with the proteomes of 11 representative yeasts revealed 2 unique and 124 expanded families of proteins in C. oleophila. Availability of the genome sequence will facilitate a better understanding the properties of biocontrol yeasts at the molecular level.
Highlights
The use of biocontrol yeasts to manage post-harvest diseases of fruits and vegetables has been actively investigated (Droby et al, 2016; Wisniewski et al, 2016; Contarino et al, 2019)
A suppressivesubtractive hybridization (SSH) cDNA library that identified several antioxidant genes associated with biocontrol activity and stress tolerance in C. oleophila I-182 was constructed (Liu et al, 2012)
The assembled gapless and near-complete genome is equivalent in length to that of the model yeast species, S. cerevisiae S288c (∼12.2 Mb3), but much less than the size of another biocontrol species M. fructicola (∼26 Mb; Piombo et al, 2018)
Summary
The use of biocontrol yeasts to manage post-harvest diseases of fruits and vegetables has been actively investigated (Droby et al, 2016; Wisniewski et al, 2016; Contarino et al, 2019). Several modes of action for the biocontrol activity of C. oleophila I-182 have been demonstrated, including competition for nutrients and space (El-Neshawy and Wilson, 1997), induction of pathogenesis-related genes and proteins (Droby et al, 2002; Liu et al, 2013), oxidative stress tolerance (Wang et al, 2018), production of extracellular lytic enzymes (Bar-Shimon et al, 2004) and superoxide anion production (Macarisin et al, 2010). The genome sequence, assembly, and annotation can be used to further elucidate the molecular mechanism underlying the biocontrol activity of yeast antagonists against several higher fungi responsible for causing decay in harvested fruits and vegetables
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