Abstract
Lysinibacillus sphaericus strain OT4b.31 is a native Colombian strain having no larvicidal activity against Culex quinquefasciatus and is widely applied in the bioremediation of heavy-metal polluted environments. Strain OT4b.31 was placed between DNA homology groups III and IV. By gap-filling and alignment steps, we propose a 4,096,672 bp chromosomal scaffold. The whole genome (consisting of 4,856,302 bp long, 94 contigs and 4,846 predicted protein-coding sequences) revealed differences in comparison to the L. sphaericus C3-41 genome, such as syntenial relationships, prophages and putative mosquitocidal toxins. Sphaericolysin B354, the coleopteran toxin Sip1A and heavy metal resistance clusters from nik, ars, czc, cop, chr, czr and cad operons were identified. Lysinibacillus sphaericus OT4b.31 has applications not only in bioremediation efforts, but also in the biological control of agricultural pests.
Highlights
Biological control of vector-borne diseases, such as dengue and malaria, and agricultural pests have been an issue of special concern in the recent years
Since Kellen et al [1] first described Lysinibacillus sphaericus as an insect pathogen, studies have shown mosquitoes to be the major target of this bacterium [2,3,4], but toxic activity against other species has been reported [5,6]
No larvicidal activity has been identified in Lysinibacillus sphaericus OT4b.31 against Culex quinquefasciatus [10]
Summary
Biological control of vector-borne diseases, such as dengue and malaria, and agricultural pests have been an issue of special concern in the recent years. Since Kellen et al [1] first described Lysinibacillus sphaericus as an insect pathogen, studies have shown mosquitoes to be the major target of this bacterium [2,3,4], but toxic activity against other species has been reported [5,6]. L. sphaericus larvicidal toxicity has been reported due to vegetative mosquitocidal toxins (Mtx) [7], the binary toxin (BinA/BinB) [4], Cry48/Cry toxin [8] and recently the S-layer protein [9]. Lysinibacillus species are potential candidates for heavy metal bioremediation. Native Colombian Lysinibacillus strains have been reported as potential metal bioremediators. The moderate heavy metal tolerance in a Lysinibacillus strain isolated from a non-polluted environment generates interest in characterizing the genomic properties of L. sphaericus OT4b.31, in addition to its biotechnological potential in biological control. We present a summary classification and a set of features for Lysinibacillus sphaericus OT4b.31 including previously unreported aspects of its phenotype, together with the description of the complete genomic sequencing and annotation
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