Abstract
Lysinibacillus sphaericus CBAM5, was isolated from subsurface soil of oil well explorations in the Easter Planes of Colombia. This strain has potential in bioremediation of heavy-metal polluted environments and biological control of Culex quinquefasciatus. According to the phylogenetic analysis of 16S rRNA gene sequences, the strain CBAM5 was assigned to the Lysinibacillus sphaericus taxonomic group 1 that comprises mosquito pathogenic strains. After a combination assembly-integration, alignment and gap-filling steps, we propose a 4,610,292 bp chromosomal scaffold. The whole genome (consisting of 5,146,656 bp long, 60 contigs and 5,209 predicted-coding sequences) revealed strong functional and syntenial similarities to the L. sphaericus C3-41 genome. Mosquitocidal (Mtx), binary (Bin) toxins, cereolysin O, and heavy metal resistance clusters from nik, ars, czc, mnt, ter, cop, cad, and znu operons were identified.
Highlights
Lysinibacillus sphaericus is one of the bacteria used as a bio-insecticide as part of vector control programs against tropical diseases, such as malaria, filariasis, yellow fever, dengue fever and West Nile virus [1]
Comparing the region comprised between the germination operon and the binA-binB genes across the sequences of L. sphaericus CBAM5, C3-41 and 2297, we found an equivalent homology of putative transposases with different length and disruption points
Lysinibacillus sphaericus CBAM5 was isolated from drilling mineral base oil samples at the subsurface soil level
Summary
Lysinibacillus sphaericus is one of the bacteria used as a bio-insecticide as part of vector control programs against tropical diseases, such as malaria, filariasis, yellow fever, dengue fever and West Nile virus [1]. We present a summary classification, and set of general features for Lysinibacillus sphaericus strain CBAM5 including previously unreported aspects of its phenotype, together with the description of its genome sequence and annotation. The applied pipeline included quality check of reads, de novo assembly, a gap-filling step and mapping against a reference genome. Insights into the genome We propose a 19-supercontig chromosomal scaffold of Lysinibacillus sphaericus CBAM5 with 4.61 Mbp in length, corresponding to a 99.4% of the reference chromosomal sequence. As per most of the variant effects, in comparison to the C3-41 strain, are allocated upstream and downstream of the gene operons, we suggest that L. sphaericus CBAM5 may enclose different regulatory elements or non-coding sequences
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