Abstract
Streptomyces spp. have been major contributors of novel natural products that are used in many application areas. We found that the nojirimycin (NJ) producer JCM 3382 has antimicrobial activity against Staphylococcus aureus via cellular degradation. Genome analysis revealed 30 biosynthetic gene clusters, including those responsible for producing antibiotics, including an azasugar NJ. In-depth MS/MS analysis confirmed the production of 1-deoxynojirimycin (DNJ) along with NJ. In addition, the production of tambromycins, setomimycin, and linearmycins was verified by spectroscopic analyses, including LC-MS and NMR. The distribution of the clusters of genes coding for antibiotics in 2061 Streptomyces genomes suggested potential producers of tambromycin, setomimycin, and linearmycin. For a DNJ gene cluster, homologs of gabT1 and gutB1 were commonly found; however, yktC1 was identified in only 112 genomes. The presence of several types of clusters suggests that different strains may produce different types of azasugars. Chemical-profile-inspired comparative genome analysis may facilitate a more accurate assessment of the biosynthetic potential to produce secondary metabolites.
Highlights
Streptomyces, the largest genus in the phylum Actinobacteria, includes aerobic, grampositive filamentous bacteria distributed in a wide range of environments, from terrestrial to marine
Recent advances in DNA sequencing technology have led to the generation of a large amount of genome sequence data from thousands of Streptomyces strains. This large volume of data provides an unprecedented opportunity to explore the genetic basis of the biosynthesis of valuable secondary metabolites produced by these organisms
The draft genome sequence of S. nojiriensis JCM 3382 was deposited in the NCBI GenBank database under accession number BMRL00000000 as a part of the Global Catalogue of Microorganisms 10K type strain sequencing project [35]
Summary
Streptomyces, the largest genus in the phylum Actinobacteria, includes aerobic, grampositive filamentous bacteria distributed in a wide range of environments, from terrestrial to marine. These bacteria have a complex life cycle, producing substrate hyphae, aerial hyphae, and spores [1]. Recent advances in DNA sequencing technology have led to the generation of a large amount of genome sequence data from thousands of Streptomyces strains This large volume of data provides an unprecedented opportunity to explore the genetic basis of the biosynthesis of valuable secondary metabolites produced by these organisms
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
