Abstract
Marine microorganisms are receiving more attention as a promising potential source of new natural products. In the present study, we performed genomic and metabolomic analyses to explore the metabolic potential of the obligate marine actinomycete genus Salinispora. The genomes of thirty Salinispora strains were prospected in search of biosynthetic gene clusters including polyketide synthase (PKS), nonribosomal peptide synthetase (NPRS), terpene, indole, lantibiotics, and siderophores. We determined considerable diversity of natural product biosynthetic gene clusters in their genome. There were a total of 1428 putative gene clusters involved in the biosynthesis of various bioactive natural products. Furthermore, 1509 ketosynthase (KS) and condensation (C) domains were detected by using NapDoS belonging to PKS and NRPS genes, respectively. Metabolic profiling was performed by a nontargeted LC-MS/MS approach combined with spectral networking using Global Natural Product Social Molecular Networking (GNPS). Dereplication and tentative identification of natural products were evaluated for common chemical properties and their associated pathways. Significant bioactive natural products such as lomaiviticin C, 7-OH-staurosporine, staurosporine, and cyanosporaside B were determined. More importantly, an unknown glycosylated compound associated with an NRPS/PKS-I hybrid gene cluster in Salinispora pacifica CNY703 was established through chemical and genomic analyses.
Highlights
Natural products are biological molecules produced by organisms such as fungi, plants, and microorganisms
Marine actinomycetes belonging to the genus Salinispora have long been an important source of structurally diverse and biologically active natural products, several of which have inspired the development of new classes of therapeutic agents (Feling et al, 2003; Fenical and Jensen, 2006; Jensen and Mafnas, 2006)
According to antiSMASH results, 1428 putative natural product gene clusters were found to be involved in the biosynthesis of various pathways such as NRPS, polyketide synthase (PKS), terpenes, butyrolactones, siderophores, bacteriocins, and lantibiotics
Summary
Natural products are biological molecules produced by organisms such as fungi, plants, and microorganisms. The biotechnological potential of natural products from microorganisms is receiving more attention for the discovery of novel bioactive compounds (Qin et al, 2017). Marine actinomycetes belonging to the genus Salinispora have long been an important source of structurally diverse and biologically active natural products, several of which have inspired the development of new classes of therapeutic agents (Feling et al, 2003; Fenical and Jensen, 2006; Jensen and Mafnas, 2006). Polyketide- and peptide-derived metabolites are among the most diverse and include many clinically important compounds (Fischbach and Walsh, 2006; Jang et al, 2013). Genomics and metabolomics have been combined to identify new bioactive metabolites. The mining of actinomycete genomes has proven to be useful in the identification of secondary metabolite biosynthetic
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