Abstract
We have previously reported the preparation of the genipin cross-linked alginate-chitosan (GCAC) microcapsules composed of an alginate core with a genipin cross-linked chitosan membrane. This paper is the further investigation on their structural and physical characteristics. Results showed that the GCAC microcapsules had a smooth and dense surface and a networked interior. Cross-linking by genipin substantially reduced swelling and physical disintegration of microcapsules induced by nongelling ions and calcium sequestrants. Strong resistance to mechanical shear forces and enzymatic degradation was observed. Furthermore, the GCAC membranes were permeable to bovine serum albumin and maintained a molecular weight cutoff at 70 KD, analogous to the widely studied alginate-chitosan, and alginate-poly-L-lysine-alginate microcapsules. The release features and the tolerance of the GCAC microcapsules in the stimulated gastrointestinal environment were also investigated. This GCAC microcapsule formulation offers significant potential as a delivery vehicle for many biomedical applications.
Highlights
Bioencapsulation describes a procedure where biologically active materials are enclosed within a semipermeable membrane [1]
The genipin cross-linked alginate-chitosan (GCAC) microcapsule had a denser and smoother surface than the AC membrane (Figures 1(c) and 1(d)), though sporadic small nubs were seen in both cases
The cumulative percentage of BSA released from the GCAC and AC capsules was 38.1% and 55.5% in the first 1 hour, respectively. Thereafter, these numbers increased to 46.8% versus 69.5% in 2 hours, and 70.4% versus 76.7% in 4 hours, and both above 95% after 12 hours (Figure 12). This delay in BSA release, which was consistent with the results obtained from the BSA ingress experiments, could be a result of transport obstruction in the GCAC membranes generated by the genipin-chitosan cross-links
Summary
Bioencapsulation describes a procedure where biologically active materials are enclosed within a semipermeable membrane [1]. This technology has proven a valuable strategy to facilitate a wide range of pharmaceutical and biomedical processes in both fundamental research and industrial applications including drug delivery, artificial organs, and cell therapy [2,3,4,5,6,7,8,9]. The key required characteristics of microcapsules for such applications include biocompatibility, adequate resistance to environmental constraints, appropriate membrane stability, and permeability [10,11,12,13,14,15,16,17]. As a follow-up study, we present the characterization of the microcapsule structure and key physical characteristics including mechanical properties, resistance, permeability, and durability
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
