Abstract
A micro-patterned cell adhesive surface was prepared for future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro-patterns were prepared by a photolithography process. Afterwards, recombinant filamentous phages that displayed a short binding motif with a cell adhesive peptide (-RGD-) on p8 proteins were immobilized on PDMS microgrooves through simple contact printing to study the cellular response of rat H9c2 cardiomyocyte. While the cell density decreased on PDMS micro-patterns, we observed enhanced cell proliferation and cell to surface interaction on the RGD-phage coated PDMS microgrooves. The RGD-phage coating also supported a better alignment of cell spreading rather than isotropic cell growths as we observed on non-pattered PDMS surface.
Highlights
For engineering the cell-material interface for controlling cell adhesion, much attention has been given to the development of various micro-patterned environments in which anisotropic design of a scaffold for biomaterials and biomedical applications are used [1,2,3]
In addition to early immune responses for extra cellular matrix proteins (ECMs), the arginine-glycine-aspartate (RGD) peptide which is recognized by integrin to promote cell adhesion has been investigated to control the cellular response on spreading, migration, morphology and orientation [13,14,15]
We investigated the effect of the RGD-phage on the cellular response in the micro-patterns of PDMS
Summary
For engineering the cell-material interface for controlling cell adhesion, much attention has been given to the development of various micro-patterned environments in which anisotropic design of a scaffold for biomaterials and biomedical applications are used [1,2,3]. -active coatings has been extensively studied [10]. Such biochemical surface modification may especially help impede early immune responses, including inflammation and encapsulation [11]. Fibrin and laminin are extra cellular matrix proteins (ECMs) and it is known that they are involved in early immune response processes such as inflammation [12]. In addition to early immune responses for ECM, the arginine-glycine-aspartate (RGD) peptide which is recognized by integrin to promote cell adhesion has been investigated to control the cellular response on spreading, migration, morphology and orientation [13,14,15]. Various experimental studies show that coating medical implants with these proteins leads to improvement in tissue integration and regeneration
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