Abstract
Herein, a micro-patterned cell adhesive surface is prepared for the future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro patterns were prepared by a photolithography process. We investigated the effect of microscale topographical patterned surfaces on decreasing the collective cell migration rate. PDMS substrates were prepared through soft lithography using Si molds fabricated by photolithography. Afterwards, we observed the collective cell migration of human lens epithelial cells (B-3) on various groove/ridge patterns and evaluated the migration rate to determine the pattern most effective in slowing down the cell sheet spreading speed. Microgroove patterns were variable, with widths of 3, 5, and 10 µm. After the seeding, time-lapse images were taken under controlled cell culturing conditions. Cell sheet borders were drawn in order to assess collective migration rate. Our experiments revealed that the topographical patterned surfaces could be applied to intraocular lenses to prevent or slow the development of posterior capsular opacification (PCO) by delaying the growth and spread of human lens epithelial cells.
Highlights
Cataract is one of the most common diseases of elderly people, and is responsible for considerable medical costs to patients
Our experiments revealed that the topographical patterned surfaces could be applied to intraocular lenses to prevent or slow the development of posterior capsular opacification (PCO) by delaying the growth and spread of human lens epithelial cells
A person with pseudophakia might experience a secondary loss of vision caused by posterior capsular opacification (PCO) that is caused by residual lens epithelial cells (LECs) left behind during cataract surgery
Summary
Cataract is one of the most common diseases of elderly people, and is responsible for considerable medical costs to patients. Time passes and the induction of ECM releases from cells and non-selective serum protein start to cover the surface of the IOL, making the environment more favorable for the cell adhesion that causes the initial PCO. Despite these discoveries, PCO remains the most common complication of cataract surgery [11]. Our experiments revealed that topographical patterned surfaces can be applied to intraocular lenses to prevent or slow the development of PCO by delaying human lens epithelial cells’ growth and spreading
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