Abstract
Assessment of genetic variations of two progenitors of S. scabra was investigated employing isozyme, RAPD and STS markers. Earlier reports have conclusively indicated S. seabrana and S. viscosa as two diploid progenitors of allo-tetraploid S. scabra. A total of 476 RAPD bands generated from 32 primers, 104 STS fragments from 17 primer pairs and 73 isoforms from 14 enzyme systems indicated significant sharing of these bands to S. scabra with that of S. seabrana in comparison to its other progenitor i.e., S. viscosa. High frequencies of isozyme band sharing between S. seabrana and S. scabra (0.125–0.30) as well as high genetic similarities (59%) of it with S. seabrana as reveled from dendrogram analysis indicated impact of close proximity of the occurrence of S. seabrana in the development of S. scabra. The proportion of bands shared by RAPD (40%) and STS (40%) markers were also observed high between S. seabrana and S. scabra. However, the numbers of unique bands were less in all these species. More correspondence and sharing of bands by S. seabrana towards S. scabra suggested using selected lines of S. seabrana the genetic base of allo-tetraploid, drought tolerant S. scabra lines can be broaden.
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