Abstract
As a nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase and ADP ribosyl transferase, the silent information regulator 7 (Sirtuin 7, SIRT7) plays a crucial role in regulating the differentiation of adipocytes and myoblasts, lipid metabolism, glucose metabolism, and cellular growth in mammals. It has been hypothesized that SIRT7 affects growth traits in animals; therefore, in this study, the potential insertion/deletion (indel) of genetic variations within the ovine SIRT7 gene and their correlation with sheep growth traits were explored. A total of 709 individuals from five Chinese and Mongolian sheep breeds were analyzed. Two novel indel loci of the sheep SIRT7 gene were detected and were named 5 promoter region-insertion-7 bp (5 promoter region-7 bp) and 3 UTR-insertion-17 bp (3 UTR-17 bp), respectively. In all of the sheep breeds, frequencies of the 5 promoter region-7 bp mutation were low, whereas mutations of 3 UTR-17 bp were high in Tong sheep and Lanzhou fat-tail sheep (LFTS). Furthermore, both indel polymorphisms had significant associations with different growth characteristics (). Among these associations, the 3 UTR-17 bp was highly correlated with rump width in small-tail Han sheep (STHS, rams; ), and individuals with the ID genotype had better chest depth values than those with the II genotype. In this paper, two novel indels within the sheep SIRT7 gene were identified, and genetic diversity and its effects on body size traits were explored. These findings will potentially provide useful DNA markers for the improvement of economic traits in sheep genetic breeding.
Highlights
Silent information regulator 2 proteins, which belonging to the class III nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylases and ADP ribosyl transferases, are connected to metabolism and life span regulation in lower organisms and are highly conservative from prokaryotes to eukaryotes (O’Callaghan and Vassilopoulos, 2017)
Studies of SIRT genes tend to focus on the regulation of the physiological processes of cell stress responses, proliferation, apoptosis, aging, metabolism, and especially on the development of tumors (Li and Kazgan, 2011; Roth and Chen, 2014; O’Callaghan and Vassilopoulos., 2017; Ye et al, 2017)
In recent years, SIRT genes have been shown to regulate the differentiation of adipocytes and myoblasts (Cioffi et al, 2015)
Summary
Silent information regulator 2 (sirtuins, SIRT2) proteins, which belonging to the class III nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylases and ADP ribosyl transferases, are connected to metabolism and life span regulation in lower organisms and are highly conservative from prokaryotes to eukaryotes (O’Callaghan and Vassilopoulos, 2017). The mammalian sirtuin proteins have been reported to participate in the regulation of energy metabolism (e.g., sugar and lipid metabolism) (Li and Kazgan, 2011; Ye et al, 2017) and stress (Blank and Grummt, 2017), as well as the maintenance of genomic stability (Bosch-Presegué and Vaquero, 2014), tumor development (Roth and Chen, 2014), cell proliferation, senescence, and apoptosis (O’Callaghan and Vassilopoulos, 2017). G.13915A>G within the SIRT4 gene significantly influences body size traits (body length, chest depth, rump length, and chest circumference) in Chinese Qinchuan cattle (Gui et al, 2016). There are few related reports regarding ovine SIRT7
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