Genetic variation of Tumour Necrosis factor in pulmonary Tuberculosis

Abstract

Tuberculosis a major cause of mortality throughout the world, therefore, early diagnosis for the prevention is needed. One of the disease control strategies is the use of molecular genetics and its role in the severity of active pulmonary tuberculosis. Identify the tumor necrosis factor-alpha (TNF-a) gene polymorphism in patients with pulmonary tuberculosis in Surabaya Pulmonary Hospital. The research recruited 97 respondents having active pulmonary tuberculosis patients. There were 52 (53.6%) mild-moderate and 45 (46.4%) TB patients. DNA isolation was performed. DNA was detected using melting curve real-time PCR Light Cycler. Differences in the nucleotide bases were confirmed with the sequencing using the ABI genetic analyzer. The allele pattern, resulting from the inspection of SNPs TNF 238 and 308 genes, was more specific with the genotype frequencies of pulmonary tuberculosis TNF-238 94(96, 91%)GG and TNF-308 90(92, 78%) homozygous GG. The overview variations of the genotyping test melting curve of TNF-857, TNF-1031, TNF-863 showed more diverse variants. The TNF-α gene variants do not directly give different clinical manifestations of pulmonary tuberculosis patients. There are TNF-α gene polymorphisms in TNF-238, -857, -863, -308 and -1031 in patients with active pulmonary tuberculosis in Surabaya Lung Hospital. TNF-α gene polymorphisms were obtained on the SNPs TNF-238, TNF-857, TNF-863, TNF-308, TNF-1031sites of patients with active pulmonary tuberculosis in Surabaya Lung Hospital. Significant correlation test existed among the five types of TNF-α gene polymorphisms, i.e. p = 0.0001, r = 27%.