Abstract
Allele frequencies for 9 autosomal short tandem repeat loci (STRs) D3S1358, VWA, FGA, THO1, TPOX, CSF1PO, D5S818, D13S317, D7S820 and a locus allowing for sex discrimination were determined in 1000 unrelated Egyptians. Blood samples were collected after obtaining an informed consent. The DNA was extracted by using the standard phenol-chloroform extraction method and then amplified using commercial available kit (AmpF L STR profiler kit, Applied Bio systems) by the polymerase chain reaction, and subsequently typed using capillary electrophoresis (3130X1 Genetic Analyser, Applied Bio systems). All loci met Hardy-Weinberg expectations and no mutations were found among subjects.
Highlights
Deoxyribonucleic acid (DNA) typing is widely used for personal identification and knowing the source of biological samples found in the crime scene [1]
Some loci have alleles differing by a number of complete repeat units, whereas others display complex polymorphism with some alleles differing in size by only 1 bp [3]
The results indicate that the examined 9 short tandem repeats (STRs) loci are useful genetic markers for forensic personal identification and paternity testing in the Egyptian Population
Summary
Deoxyribonucleic acid (DNA) typing is widely used for personal identification and knowing the source of biological samples found in the crime scene [1]. In view of their high level of variability, autosomal short tandem repeats (STRs) are very useful as markers of forensic and Population genetics studies [2]. Federal Bureau of Investigation has recommended using 13 STR loci, known as CODIS 13, as the loci of choice for forensic use. This recommendation has been accepted by forensic laboratories all over the world, the DNA typing can be compared to each other [4]. Blood samples were taken from 1000 unrelated samples form Egyptian Populations
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