Abstract
Protein content in milk is an important indicator of milk. Accordingly, genetic improvement to produce Holstein Friesian (HF) dairy cattle is important. The objective of this study was to evaluate the genetic variant of milk protein genes and its effect on milk component traits of Holstein Friesian (HF). A total of 100 HF were used in this study. The HF cattle used have physiological status in the lactation period 1 up to 3 and lactation change of 1 up to 12 months. Genotype variants of milk protein genes were identified using Real Time-Polymerase Chain Reaction method. Analysis of milk component was carried out covering the component of protein, fat, lactose, and solid non-fat (SNF) by using a milk quality measuring device (Lactoscan). Genotyping of cattle blood samples consisted of DNA extraction, genes amplification using the RT-PCR method. The result showed that<strong> </strong>protein milk was significantly affected (p&lt;0.05) by the genetic variants of CSN1S1-192 and CSN2-67 genes. Fat milk was significantly affected (p&lt;0.05) by the genetic variants of CSN1S1-192 and CSN3 genes. Meanwhile, solid non-fat milk was significantly affected (p&lt;0.05) by the genetic variants of CSN-BMC9215, CSN-BMC6334, CSN1S1-14618, CSN2_67, and CSN3 genes. Lactose milk was significantly affected (p&lt;0.05) by the genetic variants of CSN-BMC9215 and CSN2-67 genes. It was concluded that genetic variants of the milk protein genes have an association with the component of cow's milk (protein, fat, solid non-fat, and lactose).
Highlights
Genetic improvement in dairy cattle is not mainly to increase the amount of milk production and to increase milk quality which is the high milk protein content
Genetic variants of the milk protein genes have an association with the component of cow's milk
Protein milk was affected by the genetic variants of CSN1S1-192 and CSN2-67 genes
Summary
Genetic improvement in dairy cattle is not mainly to increase the amount of milk production and to increase milk quality which is the high milk protein content. Genetic improvement is needed to produce Holstein Friesian cattle which have high milk protein carrier properties. Ferretti et al (1990) revealed that the protein component of cow's milk is controlled by two family genes of casein and whey. Laible et al (2016) revealed that milk protein genes have the potential to be explored for genotypic selection to improve the cow's milk component. The aim of this study was to evaluate the genetic variants of milk protein genes and their effects on the milk component of HF cattle. Relationship of genetic variants of milk protein genes to the component traits of milk was carried out with a general linear model using SAS 9.1 software
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call