Abstract
<p>Trypanosoma evansi as an agent of Surra is one of the crucial parasitic diseases that cause great economic losses in Indonesia. These parasites need iron for growth and propagation phase which is obtained by receptor-mediated uptake of host transferin. The transferrin receptors are encoded by Expression Site Associated Genees (ESAGs). ESAG6/7 encodes transferrin receptors which reported have different affinities of a different host. The distinction of T. evansi pathogenicity is supposed to cause variability in the ESAG6/7 gene. This research was aimed to investigate the variability of genes ESAG6/7 T. evansi with different virulence in mice. This research was conducted in two steps: bioassay pathogeneicity in mice and analysis of ESAG6/7 gene sequences. The median survival time of mice was investigated after each group of mice infected by 25 T. evansi isolates from buffaloes where its geographically differ. The test results showed a difference of pathogenic virulence on 25 T. evansi isolates in mice. Sequence analysis of the ESAG6/7 gene from 25 T. evansi isolates origin from Indonesia tended to be homogeneous on the transferrin binding site but there was variability in the hypervariable site. These changes are able to separate high and low virulence of the T. evansi isolates. Phylogenetic tree analysis was formed 11 clades of 25 T. evansi. High virulence T. evansi was included in clades 7 and 10, while low virulence T. evansi was included in clade 5 and 11 and the moderate virulence was divided into those four clades.</p><p> </p>
Highlights
Trypanosomiasis caused by T. evansi has widely spread South America, Africa and Asia (Sarkhel et al 2017)
Infectivity is defined as the ability of parasite to multiply and stay alive on the host, while virulence is the capacity of parasite to damage and cause a disease
This study results showed that life time of mice in each group that infected by 25 T. evansi isolates from Indonesia was around 4-28 days
Summary
Trypanosomiasis caused by T. evansi has widely spread South America, Africa and Asia (Sarkhel et al 2017). This blood protozoon is the cause of Surra disease which is one of the crucial parasitic diseases on the animal in Indonesia. The disease has caused huge economic losses on the late case in Sumba during. Trypanosoma evansi consists of a Sawitri DH, Wardhana AH. Genetic variability of ESAG6/7 gene Trypanosoma evansi large number of morphologically identical populations that significantly different on various biological characters such as host range, virulence, pathogenicity and drug sensitivity (Sarkhel et al 2017). Genetic variability of ESAG6/7 gene Trypanosoma evansi large number of morphologically identical populations that significantly different on various biological characters such as host range, virulence, pathogenicity and drug sensitivity (Sarkhel et al 2017). Reid (2002)
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