Abstract
Aim of study: To evaluate genetic variability and population structure of C. lindemuthianum isolates in Turkey and to record the reactions of some common bean cultivars to the pathogen isolates representing different genetic groups.Area of study: The study was performed in seven provinces of Turkey.Material and methods: Genetic diversity of 91 C. lindemuthianum isolates obtained from different provinces of Turkey was characterized by 27 iPBS and 30 ISSR primers. Also, the resistance of 40 common bean cultivars was scored against three isolates representing different genetic groups.Main results: The dendrogram based on the combined dataset of iPBS and ISSR markers classified the isolates into two main groups with a genetic similarity of 72%, which closely associated with the geographic distribution of the isolates. The dendrogram of Nei’s genetic distances and Structure analysis supported the clustering of C. lindemuthianum isolates according to the geographical provinces. The results indicated that high level of genetic diversity (GST= 0.4) and low level of gene flow (NM=0.748) exist among the populations. AMOVA analysis showed that 58.7% of total genetic variability resulted from genetic differences between the isolates within populations, while 41.29% was among populations. Four cultivars showed resistant reaction to three isolates, while the other cultivars were susceptible to at least one isolate.Research highlights: The results indicated that iPBS and ISSR markers were reliable and effective tools for analyzing population structure of C. lindemuthianum and revealed high level of genetic and pathogenic diversity among pathogen populations in Turkey.
Highlights
Common bean (Phaseolus vulgaris L.) is one of the most important grain legumes for human consumption in many countries of the world
Little information is available on genotypic variability and population structure of C. lindemuthianum in Turkey
The iPBS analysis has been used for the first time to clarify intraspecific variability between the isolates of C. lindemuthianum
Summary
Common bean (Phaseolus vulgaris L.) is one of the most important grain legumes for human consumption in many countries of the world. The use of pathogen-free seed, crop rotation, fungicide treatments, and host resistance are suggested to control bean anthracnose in the disease management strategies (Mohammed, 2013). Host plant resistance is the most reliable, economical and effective method for managing anthracnose disease on bean because chemicals have negative impacts on the human or environment, and the production of disease-free seed is difficult in developing countries (Meziadi et al, 2016). The presence of many races causes the breakdown of resistance to the pathogen in cultivars carrying anthracnose resistance genes, identified by Co-symbol (Kelly & Vallejo, 2004). In Turkey, 39 races among 51 pathogen isolates were identified, 7 of which had no similarity with characterized races of C. lindemuthianum, suggesting that high level of pathogenic variability exists within the pathogen population (Madakbaş et al, 2013).
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