Abstract
Genetic variability in Colletotrichum gloeosporioides (Penz.) isolated from cassava and yam from four agro-ecological zones of Ghana
Highlights
Colletotrichum gloeosporioides (Penz.), the causal organism of anthracnose disease is a facultative parasitic fungus belonging to the Melanconiales
Of all the primer pairs used, only the ITS1 and ITS4 pair was able to amplify the genomic DNA from the Colletotrichum gloeosporioides isolates; no amplification product was observed with the internal transcribed spacer (ITS) primer pairs ITS1/ITS1-F, ITS4/ITS4-B and 5.8s/5.8SR
Using the two universal primers, ITS1 and ITS4, a unique single band PCR product between 500-600bp was obtained from amplification of the ITS regions of the C. gloeosporioides isolates (Fig. 1)
Summary
Colletotrichum gloeosporioides (Penz.), the causal organism of anthracnose disease is a facultative parasitic fungus belonging to the Melanconiales (order producing conidia in acervuli). Identification of isolates in the C. gloeosporioides has traditionally been based on morphology, shape and size of conidia, presence or absence of setae and colony growth characteristics (Smith and Black, 1990; Sutton, 1992). These characteristics can be highly variable in C. gloeosporioides and differentiation based on these characteristics may be uncertain. Kumari et al (2008) stated that pathogen variability is one of the main causes of failure to breed crop varieties or fungicidal control of plant diseases. Genetic variation of a pathogen is a pre-requisite for any resistance breeding programme
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