Abstract
AbstractA recently developed rocket immunoelectrophoretic method was used in order to estimate the variation of Rubisco protein content in fifteen (15) tobacco cultivars. The optimum time of harvest for the highest Rubisco concentration was also determined. Since polyphenols (PP) present a problem in Rubisco extraction, the polyphenol content of the same 15 tobacco genotypes was also measured, in order to identify tobacco cultivars with high Rubisco and low polyphenol content. Rubisco concentration reached its maximum value in 2‐month‐old plants and declined thereafter. At this stage significant genetic variation was recorded for Rubisco and Polyphenol content in crude leaf extracts. Four of the genotypes (KII‐S2, TA21, N34/4, and MA/ 13β) were found to be promising in respect to high Rubisco and low PP concentration. No relationship between total protein content and Rubisco concentration was recorded. Crude extract was satisfactorily preserved at the temperature of ‐70°C.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
