Abstract
Summary A procedure for the rapid production of transgenic shoots and F 1 progeny from Nicotiana clevelandii is described. Leaf discs were co-cultivated with a disarmed strain of Agrobacterium tumefaciens harbouring a binary vector system utilizing the neomycin phosphotransferase II as reporter gene. Inoculated leaf discs produced transgenic shoots at high frequency (50 %) after culturing for about three weeks on a regeneration medium which also contained 150 mg/l Kanamycin. The transgenic plants directly initiated flowering in vitro , producing a F 1 transgenic progeny. The life cycle was completed in approx. 4 months (from inoculation to F 1 progeny). Molecular analysis of the neomycin phosphotransferase marker confirmed the insertion, the expression and the transmission of the transgene to the F 1 progeny. Due to the wide use of N. clevelandii as a propagation host for most viruses the technique is of potential use in studies aimed at conferring engineered protection against viruses.
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