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Abstract
An attempt was made to utilise the recently demonstrated method of gene transfer using irradiated male gametes to transfer the B21 associated resistance to Marek's disease into a susceptible line of chickens. Although none of the 1486 progeny tested showed evidence of the transfer of the B21 haplotype a substantial proportion acquired a second marker, susceptibility to lymphoid leukosis virus. These results confirm and extend previous reports in suggesting that although gene transfer by this method occurs at a relatively high frequency, expression of the transferred genes is variable; whether this reflects properties of the DNA itself or of cellular mechanism of expression is not clear.
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