Abstract
Aureobasidium pullulans strain Y117 was transformed to hygromycin resistance using plasmid pDH33, which contains the bacterial hygromycin B phosphotransferase gene ( hph) fused to promoter elements of the Aspergillus niger glucoamylase gene ( glaA). Southern hybridizations of transformants revealed multiple, integrated copies of the vector. The glaA promoter was not induced by starch in A. pullulans as it is in A. niger; however, the transcriptional start points were the same in both species.
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