Abstract

An effective and trustworthy traceability system is important for food safety and quality; however, traditional traceability systems that only rely on the recording method do not completely prevent food fraud. DNA-based traceability techniques facilitate seamless connectivity within the entire food supply chain. A convenient and low-cost ear tag device was invented for collecting animal blood samples as an identity control, and a panel including 12 single nucleotide polymorphic (SNP) loci was selected to distinguish individuals with a matching probability of 1.70 × 10-5. The exact animal individual was identified by comparing the SNP genotype barcodes between the meat and blood samples derived from the recording system to further validate authenticity of the recording system. These results illustrate that a combination of the genetic traceability method and a traditional recording system can provide trustworthy traceability for consumers.

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