Abstract
Botryotinia fuckeliana (Botrytis cinerea) is a heterothallic ascomycete which produces linear 8-spored asci (tetrads) in apothecia. Ascospores were collected in their linear sequence from individual asci, and analysed to determine the segregation patterns of alleles at gene loci controlling mating type (MAT1) resistance to dicarboximide fungicides (Dafl) and resistance to benzimidazole fungicides (Mbcl). The alleles were present in their expected linear sequence in 58% of the asci; most irregular sequences (32% of the asci) were caused by single exchanges between adjacent spores. The MATl locus was ca 12 map units from its centromere, and the Mbcl locus showed loose linkage with its centromere. The Mbcl and Dafl loci were ca 47 map units apart on the same chromosome and not linked with the MATl locus. Pairs of homothallic ascospores were found in seven out of 105 asci. Homothallism was not caused by inclusion of more than one nucleus in ascospores, and it occurred only in ascospores predicted to carry the MATl-2 allele after meiosis.
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