Abstract
The present study presents cytological investigations of meristematic root cells of African lily (Agapanthus praecox ssp. minimus) grown in vivo and in vitro. Cellular parameters including mitotic index (MI), chromosome count and ploidy level (nuclear DNA content) were determined from 12 mm root tip segments of this species. The MI value was found to increase when cells were transferred from in vivo to in vitro conditions, perhaps due to early adaptations of cells to in vitro environment. However, addition of plant hormones in the culture medium was found to increase the MI values recorded for root cells grown in vitro. The mean chromosome number was generally stable (2n = 2x = 30) throughout the five-week culture period, indicating no occurrence of early somaclonal variation. Ploidy level analysis revealed that A. praecox root cells contained a high percentage of polyploid cells when grown in vitro after prolonged culture period. This showed that Agapanthus praecox ssp. minimus was not genetically stable...
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