Genetic relationship of clinical bacterial isolates with their environmental spreading using ERIC-PCR

Abstract

The study examined the relationship between environmental bacterial and the pathological isolates of inward patients. It identified the biological safety level procedures followed. This work was conducted during the period from December2021 till May 2022 in Ramadi city (Anbar Governorate). Samples were divided into 30 environmental samples collected from the hospital environment, sewage and water, and 100 clinical samples collected from inpatients. These patients were in The Ramadi Teaching Hospital and in The Women’s and Children Hospital. Bacterial isolates were diagnosed using biochemical tests and confirming using VITEK2 technique. Some chemical and physical variables were detected. This work studied the Genetic fingerprinting of selected bacterial species by the Enterobacterial repetitive intergenic consensus of Polymerase Chain Reaction ERIC-PCR and conducted the dendrogram program was conducted for showing the relationships between environmental and pathological isolates of E. coli. As for the genetic fingerprint examinations environmental E. coli, the statistical analysis of the dendrogram program using ERIC-PCR technique had indicated that there was a similarity ratio 74.58% with the clinical E.coli.