Effect of alcoholic Extracts of Rosmarinus officinalis and Propolis on inhibition of Staphylococcus aureus and Klebsiella pneumoniae biofilm isolate from urinary tract infection

Abstract

The present study aimed to investigate the formation of the biofilm in Klebsiella pneumonia and Staphylococcus aureus isolated from UTI patients and study the effect of natural substances (Rosmarinus officinalis and Propolis) in inhibiting the biofilm. A total of 440 urine samples were collected from patients with urinary tract infection, those who were recumbent and were referred to the Al- Ramadi Teaching Hospital, Al-Ramadi Hospital for women and children, Al-Yarmouk Hospital, and educational laboratories in the medicine city for both sexes, during the period from the 5 July, 2017 to 10 October, 2017. Specimens were diagnosed by cultured them on selective media as well as biochemical tests. Also, diagnosis was confirmed using VITEK-2 compact system. Results showed many different bacterial isolates and ratio of S.aureus isolating was (52)%13.1 , and K.pneumonia was (68)%17.1. Their sensitivity were tested against 10 antibiotics and isolates showed varying resistance depending on the type of bacteria. The susceptibility of the bacteria to produce the biofilm was detected by qualitative and quantitative methods. Also, their susceptibility of high-yielding isolates to the biofilm antibiotics resistance was investigated, preparation of the alcoholic extract of the Rosemarinus officinalis plant and Propolis and studying its inhibitory effectiveness against isolates bacteria species. The results showed that the natural materials gave a high contrast effect against Gram positive bacteria compared to Gram negative bacteria. The effect of subMIC concentration to both alcoholic extract of Rosemarinus officinalis and Propolis were tested to inhibit the adhesion of S.aureus and K.pneumonia biofilm by micro-calibration dishes. Inhibitory ratio of Rosemarinus officinalis alcoholic extract ranged between (92-94%) to S. aureus and (16-26%) to K.pneumonia. as for Propolis, inhibitory ratio ranged (68- 75%) and (9-12%) to S. aureus and K. pneumonia, respectively.