Abstract
The purpose of this work was to determine the role of the genetic component in the development of the combined course of caries and gingivitis in children. Materials and methods: The study involved 49 schoolchildren aged 7–15 years. Dental examination was carried out according to the generally accepted method. The children were divided according to the carious process activity into two groups: 0–4 (compensated caries-comparison group) and 5–8 (subcompensated caries – main group). Chronic generalized catarrhal gingivitis (СGCG) was detected in these groups with frequency: 8 (29.62 %) of 27 patients and 15 (68.18 %) of 22 patients, respectively. Biochemical analysis of the oral fluid parameters, which included an assessment of calcium, phosphorus content, the activity of acid and alkaline phosphatases, malondialdehyde (MDA), catalase, was carried out for children. Molecular genetic research was based on the genomic DNA isolation from the buccal epithelium. To determine the polymorphic variants of GSTT1, GSTM1 genes the method of multiplex polymerase chain reaction (PCR) was used and for the IL-1β (C3953T, rs1143634) and MMP13 (A77G, rs2252070) gene s– PCR followed by restriction fragment length polymorphism analysis. Results. The development chance of caries in children was associated with the GSTM1 gene deletion polymorphism in combination with the 3953CT polymorphic variant in the IL-1β gene. It was shown, that the development of caries was associated with СGCG, and this combined dental pathology was more reliably detected in patients with 3953СT and 3953TT genotypes in the IL-1β gene. Significantly increased index of malonic dialdehyde and decreased catalase activity in the presence of 3953СT and 3953TT polymorphic variants in the IL-1β gene are determined. Conclusions. Genetic predictors of increased development chance of combined dental pathology – subcompensated caries and СGCG, pathogenetically combined with increased activity and intensity of periodontal tissue damage among children, were revealed.
Highlights
The purpose of this work was to determine the role of the genetic component in the development of the combined course of caries and gingivitis in children
The children were divided according to the carious process activity into two groups: 0–4 and 5–8
To determine the polymorphic variants of GSTT1, GSTM1 genes the method of multiplex polymerase chain reaction (PCR) was used and for the IL-1β (C3953T, rs1143634) and MMP13 (A77G, rs2252070) gene s– PCR followed by restriction fragment length polymorphism analysis
Summary
У детей провели биохимический анализ ротовой жидкости, который включал оценку содержания кальция, фосфора, активности кислой и щелочной фосфатаз, малонового диальдегида (МДА), каталазы. Установлено, что вероятность развития кариеса у детей ассоциирована с наличием делеционного полиморфизма гена GSTM1 в сочетании с полиморфным вариантом 3953СT по гену IL-1β. Наличие генотипа 3953СС по гену IL-1β у детей снижало вероятность развития данной сочетанной стоматологической патологии. Установлены генетические предикторы, повышающие вероятность развития сочетанной стоматологической патологии – субкомпенсированного кариеса и ХГКГ, которые патогенетически связаны с повышением активности и интенсивности поражения тканей пародонта у детей. У дітей виконали біохімічний аналіз ротової рідини, який включав оцінювання вмісту кальцію, фосфору, активності кислої та лужної фосфатаз, малонового діальдегіду (МДА), каталази. Встановили, що вірогідність розвитку карієсу в дітей асоційована з наявністю делеційного поліморфізму гена GSTM1 в поєднанні з поліморфним варіантом 3953СT за геном IL-1β. Визначили вірогідне підвищення показника малонового діальдегіду та зниження активності каталази за наявності поліморфних варіантів 3953СТ і 3953ТТ за геном IL-1β
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