GENETIC MARKERS OF METABOLIC DISORDERS AFTER RADICAL HYSTERECTOMY AND OOPHORECTOMY

Abstract

In 38 operated patients with benign uterine diseases, represented by such isolated forms as endometrial hyperplasia, uterine myomas and adenomyosis, as well as their combination, a clinical assessment of the quantitative indices of fat metabolism disorders, such as the average value of the body mass index (BMI) before treatment, 3, 6 and 12 months after surgery, as well as changes in blood pressure (BP). Clinical and laboratory analysis and analysis of genetic markers made it possible to identify alleles of high risk of arterial hypertension, as well as disorders of fat metabolism and the formation of metabolic syndrome (MS), to conclude that the identified candidate alleles allow predicting changes in BMI, BP and to form risk groups, to take preventive measures to prevent the formation of complications such as diabetes, myocardial infarction and stroke in overweight patients who have undergone surgery. The aim of the work is to identify prognostically significant genetic markers for the development of arterial hypertension, disorders of fat metabolism, and MS after subtotal hysterectomy. Material and methods. There were examined 38 patients after subtotal hysterectomy with bilateral adnexectomy or without it. Within 12 months after surgery, changes in BMI and systolic BP were observed in dynamics. The polymorphism of the following genes was studied in all patients in the studied groups: s.388T> C and p.526 C> T in the ApoE gene, pp. 306-109-2306-108insA288 in the ACE gene, p.176T> C in the IGBT3 gene. DNA samples obtained by the method of phenol-chloroform extraction from 10 ml of whole venous blood served as the material for the study of polymorphisms. Analysis of single nucleotide polymorphisms of genes (SNP) was performed by the method of minisequencing followed by mass spectrometric fractionation of oligonucleotide probes using time-of-flight (MALDI-TOF) mass spectrometry. Results. Specific alleles of the ACE and ApoE genes, characteristic of a high risk of developing hypertension, as well as prognostically significant candidate alleles of MS development, and the likelihood of impaired fat metabolism were established.