Genetic linkage map construction and QTL mapping of salt tolerance traits in Zoysiagrass (Zoysia japonica).

Hailin Guo,Yiqi Zheng,Zhiyong Wang,Wanwen Ding,Xuan Chen,Jingbo Chen,Jianxiu Liu

doi:10.1371/journal.pone.0107249

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Journal: PLoS ONE	Publication Date: Sep 9, 2014
Citations: 35	License type: CC BY 4.0

Affiliation: Institute of Botany, Chinese Academy of Sciences

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Zoysiagrass (Zoysia Willd.) is an important warm season turfgrass that is grown in many parts of the world. Salt tolerance is an important trait in zoysiagrass breeding programs. In this study, a genetic linkage map was constructed using sequence-related amplified polymorphism markers and random amplified polymorphic DNA markers based on an F1 population comprising 120 progeny derived from a cross between Zoysia japonica Z105 (salt-tolerant accession) and Z061 (salt-sensitive accession). The linkage map covered 1211 cM with an average marker distance of 5.0 cM and contained 24 linkage groups with 242 marker loci (217 sequence-related amplified polymorphism markers and 25 random amplified polymorphic DNA markers). Quantitative trait loci affecting the salt tolerance of zoysiagrass were identified using the constructed genetic linkage map. Two significant quantitative trait loci (qLF-1 and qLF-2) for leaf firing percentage were detected; qLF-1 at 36.3 cM on linkage group LG4 with a logarithm of odds value of 3.27, which explained 13.1% of the total variation of leaf firing and qLF-2 at 42.3 cM on LG5 with a logarithm of odds value of 2.88, which explained 29.7% of the total variation of leaf firing. A significant quantitative trait locus (qSCW-1) for reduced percentage of dry shoot clipping weight was detected at 44.1 cM on LG5 with a logarithm of odds value of 4.0, which explained 65.6% of the total variation. This study provides important information for further functional analysis of salt-tolerance genes in zoysiagrass. Molecular markers linked with quantitative trait loci for salt tolerance will be useful in zoysiagrass breeding programs using marker-assisted selection.

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Soil salinity is an escalating problem worldwide that affects 10% of the land area of the world, which amounts to 1 billion hectares [1]
In this study we developed a genetic linkage map for a population derived from an intraspecific cross between two Z. japonica germplasms (Z105 and Z061) using sequence-related amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) markers, and reported a mapping of quantitative trait locus (QTL) affecting salt tolerance
Marker polymorphism In this study, we found that the SRAP markers detected more polymorphisms in zoysiagrass than the RAPD markers

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Introduction

Soil salinity is an escalating problem worldwide that affects 10% of the land area of the world, which amounts to 1 billion hectares [1]. The growth of salt tolerant plants is an effective and economic way to reduce the spread of salinization and to make full use of land resources. Though most of this land is currently too saline for conventional agriculture, a good proportion of it has the potential to be used for growing salt tolerant grasses [3]. Zoysiagrass (Zoysia Willd.) is an important warm season turfgrass that is indigenous to countries on the western Pacific Rim and westward into the Indian Ocean [4]. Numerous studies have assessed the effects of salinity on zoysiagrass growth and salinity tolerance [8,9,10,11,12,13]. In breeding for salt tolerance, DNA marker-assisted selection is useful to select inherited genetic markers that are associated with the trait, and to use them as indirect selection criteria for marker-assisted breeding

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