Abstract
Burning of corpses is a well-known and widely spread funeral procedure that has been performed for a long time in many cultures. Nowadays more and more corpses are burned in cremations and buried in urns, often for practical and financial reasons. Usually cremation takes place as temperatures of over 1000 °C for more than 70 min, destroying the corpse and only leaving severely burned teeth and some fragments of larger bones. In some scientific, criminal or civil cases even after cremation, there is the need of genetic investigations for identification or paternity testing. Here we present a systematic genetic investigation of 10 corpses that were burned in a crematory. After DNA extraction of the remains, the presence of human nuclear and mitochondrial DNA was tested by a highly sensitive Duplex PCR and quantified via real time PCR; genetic typing was done using the AmpFlSTR Identifiler kit.
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