Abstract
The development of animal husbandry suffers various kinds of losses due to the spread of infectious diseases among animals, in particular Brucellosis. A challenge faced by Brucella researchers has been the accurate identification of new isolates within the genus while preserving sufficient, and not excessive, biosafety and biosecurity requirements. The availability of discriminatory molecular typing tools to inform and assist conventional epidemiological approaches would be invaluable in controlling these infections, but efforts have been hampered by the genetic homogeneity of the genus. In this work, for better identification of infection, for control and monitor the source of outbreaks in prosperous areas was carried out identification of Brucella spp. strains which circulating in the Kostanay region. For this was used using multilocus analysis of a variable number of tandem repeats sequenced by 16 s – PNK on a genetic analyzer (sequencer). According to the results of a study of cattle, cultures of microorganisms were infected: No. 4, 5, 7, 8. Comparison of the obtained results with similar results of domestic and foreign works by A. Shevtsov, G. Borrello, P. Le Fleche, G. Garofolo suggest that the genotyping of local strains has an importance in the molecular epizootology of the Republic of Kazakhstan.
Highlights
Brucellosis is a zoonotic disease of major public health, animal welfare, and economic significance worldwide
The availability of discriminatory molecular typing tools to inform and assist conventional epidemiological approaches would be invaluable in controlling these infections, but efforts have been hampered by the genetic homogeneity of the genus
Among the 12 species currently proposed in this genus, Brucella melitensis, Brucella abortus, and Brucella suis are highly dangerous for humans and cause disease with severe complications and chronic processes (Meyer, 1990; Godfroid et al, 2011)
Summary
Brucellosis is a zoonotic disease of major public health, animal welfare, and economic significance worldwide. Infection with Brucella can lead to a chronic debilitating infection; in domesticated animals, the main symptom is a reproductive failure. Among the 12 species currently proposed in this genus, Brucella melitensis, Brucella abortus, and Brucella suis are highly dangerous for humans and cause disease with severe complications and chronic processes (Meyer, 1990; Godfroid et al, 2011). Brucellosis causes great economic damage to livestock. The disease is accompanied by mass abortions and barrenness of animals suffering from brucellosis, joint damage, inflammation of mucous bags (in horses at the withers and the nape of the neck), the sex glands, the appearance of abscesses (more often in pigs), a decrease in the viability of offspring, a decrease in animal productivity. The development of discriminatory molecular tools for the identification and typing of Brucella has been problematic, reflecting the lack of genetic polymorphism in Brucella
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