Genetic Fidelity Studies using RAPD Marker on local fungal isolate of Jabalpur Region for over-expression of Glucoamylase

Abstract

The present study was conducted to study genomic profile of wild and mutant isolates of local soil fungal isolate Aspergillus flavipes strain for over-production of glucoamylase using RAPD markers. The study involves application of mutational strategies using chemical and physical mutagens on the potent fungal isolate and their screening studies thereafter. The alteration in genetic makeup of the mutant fungal isolate (SSP#16M) with the wild type (SSP#16) was detected and confirmed by RAPD-PCR analysis of genomic DNA. Although various genotypic methods have been used for comparison of Aspergillus species but no comparable data is available for Aspergillus flavipes strain for increased enzyme production. In the study, the mutant fungal isolate designated NA3 (exposed to nitrous acid) showed 3.5 fold improved glucoamylase activity than wild isolate and showed consistency in the production of the enzyme after repeated subcultures. The data for genetic variability was analysed using software Dendro UPGMA wherein the similarity matrix was computed with Jaccard coefficient. This assessment study of mutant isolate of Aspergillus flavipes strain provides potential applicability in starch processing industries.