Abstract
BackgroundBletilla striata is a well-known traditional Chinese herb with varieties of functions. In China, the natural resources of Bletilla striata have been severely damaged because of the excessive exploitation and destruction of natural habitats. The aim of present study was to provide a reference for fully exploiting and utilizing the germplasm resources of Bletilla striata.ResultsThe genetic diversity of 50 varieties of Bletilla striata from different area in China was analyzed by SCoT and IRAP molecular marker technique. A total of 209 bands were amplified by 20 groups of SCoT primers, of which 201 (96.17%) were polymorphic, and 47 polymorphic bands (94%) were observed in 50 bands amplified by 8 groups of IRAP primers. The 50 populations of Bletilla striata were divided into two major groups by SCoT and IRAP at the genetic similarity coefficient value of 0.60 and 0.68 individually. The partition of clusters in the unweighted pair group method with arithmetic mean dendrogram and principal coordinate analysis plot based on the SCoT and IRAP markers was similar.ConclusionsResults indicated the abundant genetic diversity of Bletilla striata among different areas. Our results will provide useful information for resource protection.
Highlights
Bletilla striata is a well-known traditional Chinese herb with varieties of functions
Genetic diversity revealed by start codon targeted (SCoT) polymorphic markers Twenty groups of primers were used to detect genetic polymorphisms in the samples
The number of the polymorphic bands ranging from 6 to 13
Summary
Bletilla striata is a well-known traditional Chinese herb with varieties of functions. In China, the natural resources of Bletilla striata have been severely damaged because of the excessive exploitation and destruction of natural habitats. Bletilla striata is a well-known traditional Chinese herb, which was first described in Shennong BenCao Jing (Shennong’s Materia Medica) 2000 years ago. Traditional Chinese Medicine holds that Bletilla striata is capable of restraining blood leakage, stopping bleeding, dispersing swelling and promoting tissue regeneration. It could be effectively applied in the treatment of hematemesis, hemoptysis, traumatic bleeding, chapped kin, and ulcerative carbuncle [4, 17]. The non-medical uses of Bletilla striata include rubbing its mucilaginous roots in inkstones
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call