Abstract
Pastures have over the years been selected for important traits and their breeding has resulted in some high yielding forage grasses with great adaptive and population expansion traits. The research was carried out to study genetic diversity of nine Andropogon spp. sourced from Remo zone of Ogun State using four microsatellite markers (Phil227562, Xcup63, CTM59, and Xcup14). Genomic DNA was extracted from succulent leaf part of Andropogon grass using DNA extraction procedure of Zymo spin™ technology. Every one of the loci–populace is in with Hardy–Weinberg equilibrum. A sum of eight alleles were noticed and the quantity of alleles per locus has an estimation of 2.00, while the number of alleles ranged from 1.528 (Phil227562) to 1.946 (Xcup63). The viable number of alleles was lesser than noticed number at all the loci. The Shannon's Information Index differed among the loci with 0.530 (Phil227562) to 0.679 (Xcup63) though Inbreeding coefficient across the Andropogon grass clones was negative (mean = - 0.429), going from 0.07 (Phil 227562) to 0.714 (Xcup63) with 100% polymorphic loci. The noticed heterozygosity was higher than expected heterozygosity for all markers of the grass considered.
Highlights
Sampling sites Fresh leaf portions of young Andropogon grasses were harvested from nine geographical locations in Remo zones of Ogun State (Table 1). 5g of the leaf parts were prepared in ethanol before DNA extraction study
Genotypic difference of microsatellite markers used for Hardy Weinberg Test In the population of Andropogon spp. collected at Remo, the genotype AB of locus Phil227562 and Xcup 63 has the highest frequency of 0.833, for locus Xcup14 genotype AB has the lowest frequency of 0.6 while the genotype BB of locus CTM59 has the highest frequency (0.56), the genotype AB has low genetic variation in its loci which might provide adequate information on genetic selection of Andropogon spp. (Table 3)
A high hereditary variety was seen among the grass species used which is ascribed to recombination as a result of heterozygosity observed in the species (Azevedo et al, 2012)
Summary
Grasses are generally the most utilized feed for animals in ruminant production. Comparative qualities have been carried out to evaluate the significance of grasses, and since they share a comparative supplement of qualities, similar arrangement of qualities may have been chosen (Edward et al, 2001) leading to some other important genes being neglected. Eighties which gained surprising ground at the worldwide level These different biotechnological instruments incorporated procedures for understanding the hereditary construction of the plants, incorporating unfamiliar qualities directly into the plant genome and in-vitro recovery of plants from any plant part (Ajoy et al, 2009). Protection of hereditary assets involves a few exercises, a significant number of which may enormously profit by information produced through applying micro satellite marker advancements. This is a technique associated with identifying and securing germplasm (finding and depicting the variety), its preservation (utilizing compelling systems) and assessment for helpful attributes. The aim of this research, was to decide the hereditary variety of Andropogon spp. using four microsatellite markers for better improvement
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
