Abstract

Aegilops is known as the ancestor species of wheat and has a major role in the evolution of wheat. Considering the importance of this species and the fact that Iran is the center of the distribution, fifteen IRAP and REMAP markers were used to evaluate the genetic diversity of Ae. tauschii accessions from different regions of Iran and its relationship with Triticum durum and Triticum aestivum. A high level of polymorphism (99%) was observed for both retrotransposon markers. Polymorphism information content, the effective number of alleles (Ne), Nei’s genetic diversity (H), and Shannon’s information index (I) for IRAP markers were more than REMAP markers. The most genetic diversity was detected in Ae. tauschii accessions. The cluster analysis separated the accessions into three distinct groups. Ae. tauschii accessions from Golestan province grouped in the same cluster with T. aestivum genotypes, indicating that these accessions are probably more like to the progenitor of the bread wheat D genome. Our results revealed that retrotransposon-based molecular markers could be used as the appropriate and reliable marker system to evaluate genetic diversity and relationship in Triticum and Aegilops accessions.

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