Abstract
The two taxa, Narcissus poeticus and N. radiiflorus, although apparently quite morphologically distinct, grow together and there has been some debate as to whether they should be considered as a single or two separate species. In order to throw some light on this debate, a survey of amplified fragment length polymorphism (AFLP) was conducted to examine genetic diversity/similarity among 120 individuals of N. poeticus and N. radiiflorus ob- tained from two populations coming from sites separated by more than 100 km. In addition, a karyological study of the two taxa was carried out to determine chromosome number and morphology, the number of nucleoli and band- ing pattern with the fluorochromes chromomycin A3 and DAPI. Using two AFLP primer pairs, a total of 103 clearly scorable fragments were amplified; of which 91 (88%) were polymorphic (78% if polymorphism is described as the most common band not present in more than 95% of individuals sampled). Cluster analysis and principal coordi- nates analysis of the calculated similarity matrix revealed there to be only a low level of genetic diversity between the entities constituting the populations and species. The genetic distance between the individual belonging to the dif- ferent populations of the same species was slightly greater than that between species. Comparative chromosome analysis showed no differences in the two taxa at the cytological level.
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