Genetic Diversity in Peripheral Pedunculate Oak (Quercus robur L.) Provenances—Potential Climate Change Mitigators in the Center of Distribution despite Challenges in Natural Populations

Abstract

Croatian pedunculate oak (Quercus robur L.) populations represent southern range peripheral populations, often viewed as sources of valuable diversity and drought-resistant ecotypes. At the same time, they endure stronger selection pressures as a result of climate change. The leaves of 20 individuals per population (17) were sampled in a field trial and analyzed using 10 nuclear and 9 chloroplast SSRs to determine the level of intrapopulation genetic variability and genetic differentiation. Analysis with nSSRs revealed deviation from HWE in seven populations. AMOVA showed a high intra-population diversity (98.53%) and a small but statistically significant inter-population differentiation. Isolation by distance explained 19.6% of differentiation. Average FST between populations was low (0.013) compared with usual values for peripheral populations. Populations were rich in cpSSR haplotypes, confirming the hotspot of diversity caused by the encounter of recolonization routes. Unbiased haplotype diversity (HE) from 9 chloroplast SSRs and 325 individuals was (HE = 0.440). Sixty-six different haplotypes were grouped in three maternal lineages by both a median-joining network and a neighbor-joining algorithm. AMOVA for cpSSRs showed statistically significant diversity among populations (70.23%), suggesting genetic differentiation, but also a probable anthropogenic effect. AMOVA of nSSRs within and between lineages showed that original recolonization patterns of nuclear diversity were subsequently erased by gene flow.