Abstract
Low productivity of pilosae type lentils grown in South Asia is attributed to narrow genetic base of the released cultivars which results in susceptibility to biotic and abiotic stresses. For enhancement of productivity and production, broadening of genetic base is essentially required. The genetic base of released cultivars can be broadened by using diverse types including bold seeded and early maturing lentils from Mediterranean region and related wild species. Genetic diversity in eighty six accessions of three species of genus Lens was assessed based on twelve genomic and thirty one EST-SSR markers. The evaluated set of genotypes included diverse lentil varieties and advanced breeding lines from Indian programme, two early maturing ICARDA lines and five related wild subspecies/species endemic to the Mediterranean region. Genomic SSRs exhibited higher polymorphism in comparison to EST SSRs. GLLC 598 produced 5 alleles with highest gene diversity value of 0.80. Among the studied subspecies/species 43 SSRs detected maximum number of alleles in L. orientalis. Based on Nei’s genetic distance cultivated lentil L. culinaris subsp. culinaris was found to be close to its wild progenitor L. culinaris subsp. orientalis. The Prichard’s structure of 86 genotypes distinguished different subspecies/species. Higher variability was recorded among individuals within population than among populations.
Highlights
The cultivated lentil (Lens culinaris subsp. culinaris) is annual, diploid (2n = 2x = 14) with genome size of ~4 Gbp [1]
Mean maximum number of effective alleles were present in L. culinaris subsp. orientalis (1.549) followed by L. culinaris subsp. culinaris (1.455) and L. ervoides
We investigated the genetic diversity and population structure among three Lens species using EST-simple sequence repeats (SSRs) and Genomic SSRs
Summary
Culinaris) is annual, diploid (2n = 2x = 14) with genome size of ~4 Gbp [1]. This crop was domesticated in Near East approximately 10,000 years ago [2]. The scientific name Lens culinaris was given by Medikus in 1787 [3]. The genus Lens includes both cultivated and wild forms distributed in West Asia and North Africa. Wild forms are confined to Mediterranean region. The genus Lens comprises seven taxa in PLOS ONE | DOI:10.1371/journal.pone.0138101. The genus Lens comprises seven taxa in PLOS ONE | DOI:10.1371/journal.pone.0138101 September 18, 2015
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