Genetic Diversity and Molecular Phylogeny of Iranian Goats Based on Cytochrome Oxidase I (COXI) Gene Sequences (KERAGAMAN GENETIK DAN FILOGENI MOLEKULER KAMBING-KAMBING IRAN BERDASARKAN SEKUENS GEN CYTOCHROME OXIDASE I (COXI))

Hamid Reza Seyedabadi,Reza Seyed Sharifi,Sima Savar Sofla

doi:10.19087/jveteriner.2017.18.4.565

Abstract

Mitochondrial DNA has been one of the most widely used molecular markers for phylogenetic studies in animals because of its simple genomic structure. This study examines the genetic characteristic of domestic goat using sequence analysis of mitochondrial DNA Cytochrome oxidase subunit I (COXI) to identify and differentiate among three common breeds (Adani, Najdi and Markhoz) of Iran. The genomic DNA was isolated by salting out method and amplified cytochrome oxidase I gene using Polymerase Chain Reaction (PCR) method with a pair of primer. Phylogenetic trees and pairwise calculations were obtained by using Mega 6 software. A partial sequence of cytochrome oxidase I gene of Iranian goats is 1286 bp and contained four variable sites and three haplotypes. Phylogenetic analysis of haplotype in the combination with the goat from GenBank showed that Iranian goatclustered in a separate lineage. This study was found informative for establishing relationships between breeds from different parts of the world. This study may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country.

Highlights

Goat was the first animal to be domesticated by humankind
Percentage of nucleotide pairs A+T was 58% and the C+G was 42%, suggesting that A+T nucleotides were higher in the Cytochrome oxidase subunit I (COXI) gene of mtDNA Iranian goat breeds
The present study is the first example of a C Oxidase Subunit 1 (COX1) phylogenetic analysis of Iranian goat breeds evaluated at the maximum level of resolution

Summary

Introduction

Goat was the first animal to be domesticated by humankind Since they have small size, the power of high compatibility, low expectations and high resistance against many diseases and hard environmental conditions has led to the preservation and breeding of these animals. The small breed of goat is distributed over the western and NorthWest of Iran near to the Turkey and Iraqi borders. They produce quite fine Mohair with different colors e.g. white, golden, brown, gray and even black. Najdi is a breed of domestic goat native to the Khozestan providence of the Iran. They have long with drooping ears and short and soft hairs. Though its meat may be consumed locally, it is especially valued for its milk

Objectives

Methods

Results

Conclusion