Abstract
Prairie grass (Bromus catharticus Vahl) is an important grass species that could be used in the production systems of certified seed and high-quality forage for grazing ruminants. In the present research, a sequence-related amplified polymorphism (SRAP) marker was employed to detect the genetic variability and structure of 80 prairie grass accessions from all over the world. Altogether, 460 reliable bands were amplified from 47 SRAP primer pairs with 345 (75%) polymorphic bands. The average values of discrimination power (DP) and polymorphic information content (PIC) were 0.753 and 0.317, respectively. Both the UPGMA clustering and PCoA analyses grouped the 80 accessions into five clusters, whereas the STRUCTURE analysis showed that 80 prairie grass accessions possessed three genetic memberships (K = 3). The results of the Mantel test showed that the distance matrix has a moderately positive correlation between the morphological and molecular data sets (r = 0.524). A poor genetic differentiation (Fst = 0.045) was discovered among the six geo-groups of accessions. Besides, the highest intragroup genetic diversity was found in the North America group (He = 0.335). This study provides a genetic structure and diversity case for prairie grass, and supplies new clues for the study and utilization of prairie grass.
Highlights
The genus Bromus belongs to the tribe Bromeae which is part of subfamily Pooideae of the family Poaceae and consists of approximately 150–160 annual and perennial species of grasses that are distributed in temperate regions of the globe [1]
Genetic diversity among all assayed 80 accessions of prairie grass were assessed through 47 different sequence-related amplified polymorphism (SRAP) primer combinations
We evaluated the genetic diversity and molecular characterization of worldwide prairie grass accessions using SRAP markers
Summary
The genus Bromus belongs to the tribe Bromeae which is part of subfamily Pooideae of the family Poaceae and consists of approximately 150–160 annual and perennial species of grasses that are distributed in temperate regions of the globe [1]. Prairie grass is classified in the section Ceratochloa of genus Bromus and is a hexaploid species (2n = 6x = 42) with the genomic formula AABBCC [1] This species is recognized as an important grass resource in producing high-quality forage for the production systems of grazing ruminants and in the production of certified seed. B. catharticus is potentially useful in soil and water conservation and cultivated pasture improvement due to its strong regeneration and adaptability to diverse environmental conditions
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
