Abstract
Simple SummaryThe Tsushima leopard cat, Prionailurus bengalensis euptilurus, is a small regional population of the Amur leopard cat and is only found on Tsushima Island in Japan. A breeding program will require adequate information on parentage, kinship, and inbreeding for this population. Hence, there is an urgent need to develop this information in order to conserve the population and its genetic diversity. We performed GRAS-Di analysis to investigate the genetic diversity and genetic structure of the Tsushima leopard cat. We identified between 133 and 158 single-nucleotide polymorphism (SNP) markers in three different genotyping methods. These SNP markers can be used in identification of individuals and parentage. In addition, structure analysis using these markers demonstrated the similar genetic composition of the samples from 48 Tsushima leopard cats, and indicated Tsushima leopard cats have no subpopulations. We have provided genetic markers that are useful for conservation of the Tsushima leopard cat, such as individual identification and parentage. Moreover, we have also clarified units for conservation of the Tsushima leopard cat population from structure analysis.The Tsushima leopard cat (Prionailurus bengalensis euptilurus) lives on Tsushima Island in Japan and is a regional population of the Amur leopard cat; it is threatened with extinction. Its genetic management is important because of the small population. We used genotyping by random amplicon sequencing-direct (GRAS-Di) to develop a draft genome and explore single-nucleotide polymorphism (SNP) markers. The SNPs were analyzed using three genotyping methods (mapping de novo, to the Tsushima leopard cat draft genome, and to the domestic cat genome). We examined the genetic diversity and genetic structure of the Tsushima leopard cat. The genome size was approximately 2.435 Gb. The number of SNPs identified was 133–158. The power of these markers was sufficient for individual and parentage identifications. These SNPs can provide useful information about the life of the Tsushima leopard cat and the pairings and for the introduction of founders to conserve genetic diversity with ex situ conservation. We identified that there are no subpopulations of the Tsushima leopard cat. The identifying units will allow for a concentration of efforts for conservation. SNPs can be applied to the analysis of the leopard cat in other regions, making them useful for comparisons among populations and conservation in other small populations.
Highlights
The leopard cat (Prionailurus bengalensis) is widely distributed throughout South Asia, East Asia, and Southeast Asia
Several genetic studies have been performed, and the results have indicated that the Tsushima leopard cat has less genetic variation than other Asian leopard cat populations [5,6,7]
Using genotyping by random amplicon sequencing-direct (GRAS-Di) analysis, 71,634,742 raw reads with an average of 1,492,578 reads per sample were obtained for 48 individuals (DDBJ Accession Number: BIMV01000001-BIMV01065356)
Summary
The leopard cat (Prionailurus bengalensis) is widely distributed throughout South Asia, East Asia, and Southeast Asia. The Leopard cat is classified as Least Concern by The International Union for Animals 2020, 10, 1375; doi:10.3390/ani10081375 www.mdpi.com/journal/animals. Conservation of Nature (IUCN) Red List of Threatened Species [1]. The leopard cat is generally classified into 12 subspecies [2]. A recent genetic study suggested that the leopard cat has only four subspecies [3]. The Amur leopard cat (Prionailurus bengalensis euptilurus), which is one of the subspecies, is distributed in Far East Asia. The Tsushima leopard cat is found only in Tsushima, Japan (Figure 1)
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