Genetic diversity and biochemical analysis of Capsicum annuum (Bell pepper) in response to root and basal rot disease, Phytophthora capsici

Abstract

This study analyzed the genetic variability and biochemical characteristics of edible and ornamental accessions of pepper, Capsicum annuum, in response to root and basal rot disease (RCR), caused by Phytophthora capsici, using resistance screening and genetic variability via Inter Simple Sequence Repeat marker (ISSR), bio-mass parameters, and enzymatic activity of Peroxidase or peroxide reductases (POX), Superoxide dismutase (SOD), Polyphenol oxidase (PPOs), Catalase (CAT), Phenylalanine ammonia-lyase (PAL), β-1,3-glucanase and phenolic content. The resistance in C. annuum ‘37ChilPPaleo’, ‘19OrnP-PBI’ and ‘23CherryPOrsh’ and susceptibility in ‘2BP-PBI′, ‘24BP-301’ and ‘26BPRStarlet’ accessions were confirmed. Nineteen out of 21 ISSR primers generated 185 polymorphic bands with a mean percentage band of 98.5 %, and an average number of bands of 9.9 per primer. Biomass parameters were significantly higher in resistant genotypes than the susceptible ones and non-inoculated controls. All the seven candidate enzymes were highly up-regulated in the resistant C. annuum accessions ‘19OrnP-PBI’, ‘37ChillP-Paleo’ and ‘23CherryP-Orsh’ inoculated with P. capsici The mean level of enzyme activity varied from 1.5 to 5.6-fold higher in the resistant C. annuum, of which SOD was increased by 5.6 fold, followed by PAL 4.40 and PPO 3.75 fold in comparison to susceptible and non-inoculated controls. Overall, there was no significant correlation between resistance and genetic variability, and also between genetic variability and enzyme activity levels. However, there was a highly significant correlation between the resistance, bio-mass parameters and enzyme activity levels.