Abstract
Two species of marine catfish, Arius maculatus and Osteogeneiosus militaris, belonging to family Ariidae were analysed electrophoretically for genetic variation in 6 enzymes, alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), lactate dehydrogenase (LDH), glucose dehydrogenase (GDH), malic enzyme (ME) and superoxide dismutase (SOD). Eighteen individuals of each species were studied. Two loci MDH and ADH were polymorphic in both. Average heterozygosity in A. maculatus was 1.46, while it was 2.5 in O. militaris. The allele frequencies were used to estimate Nei's genetic distance (D). The D value was calculated to be 0.6879. Two isozyme loci, ME and SOD, were found to be the most reliable species specific markers. No tissue specific loci were observed for the enzymes studied, the bands being identical in each case. The genetic distance observed between O. militaris and A. maculatus in this study suggests that they would be more appropriately classified as species of the same genus rather than being assigned separate genera. (Asian-Aust. J. Anim. Sci. 2004. Vol 17, No. 9 : 1188-1191)
Highlights
Allozymes profiles yield useful information about genetic divergence among species and have been used to distinguish between the closely related species (Rognon et al, 1998; Mo et al, 2003)
The total length of the specimens analysed ranged between 7-8 cm in A. maculatus and 20-22 cm in O. militaris
The tissue was homogenised in 10 mM Tris/1 mM EDTA buffer, pH 6.8, and directly subjected to native polyacrylamide gel electrophoresis (PAGE) for phenotypic analysis
Summary
Allozymes profiles yield useful information about genetic divergence among species and have been used to distinguish between the closely related species (Rognon et al, 1998; Mo et al, 2003). The total length of the specimens analysed ranged between 7-8 cm in A. maculatus and 20-22 cm in O. militaris. Isozyme profiles were developed for ADH, LDH, MDH, GDH, SOD and ME, using the staining procedures given by Pasteur et al (1988).
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