Abstract
The Mediterranean-Aegean region play a significant role in the context of conserving biodiversity. The protection of endemic and local species becomes attainable only with a thorough understanding of their biology. Specifically, aquatic species dwelling in inland waters may be more vulnerable due to the irregular composition of their habitats, characterized by natural barriers. It becomes imperative to initiate the conservation process by identifying the fauna and flora of ecosystems, thereby facilitating the development of comprehensive conservation plans. In this context, the primary objective of the present study is to identify freshwater fish species inhabiting Madra and Havran Streams in Balıkesir, Türkiye using the DNA barcoding method. The procedure involves DNA isolation through the Chelex protocol, followed by the amplification of the mitochondrial CO1 region using various primer combinations. The results obtained from the gene sequences of 29 individuals in total provide valuable information on species diversity, genetic relationships, and variations. This research emphasizes the importance of DNA barcoding as a valuable tool for species identification, genetic exploration, and conservation plans. The acquired outcomes establish a foundation for the effective management of aquatic biodiversity, particularly within these vulnerable ecosystems.
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