Abstract
The cowpea mosaic virus strain Vu (CPMV-Vu) contains two centrifugal nucleoprotein components, designated bottom and middle, whose nucleic acids interact to enhance infectivity over the individual components. Two local-lesion mutants of CPMV-Vu were isolated following nitrous acid treatments. One of the mutants had an altered nucleoprotein component ratio. Mixed component (MC) isolates arising from an interaction of homologous and all possible heterologous mixtures of components were obtained from the infectivity dilution-end-point of systemic-host assays. The local lesion types and component ratios of the MC isolates were determined. In all but two cases, the dominant factor for lesion type and nucleoprotein component ratios resided in the bottom component RNA. The results suggest that the lesion types observed were controlled by an interaction of the gene products from both component RNAs. The components of one MC isolate were backcrossed with the wild and mutant isolate components. The properties of the backcrossed MC isolates indicate that genetic complementation occurs between the nucleic acids of the two nucleoprotein components.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call