Genetic characterization of H1N2 influenza a virus isolated from sick pigs in Southern China in 2010

Wei Li Kong,Pei Rong Jiao,Gui Hong Zhang,Liang Zong Huang,Heng Wang,Nan Cao,Shang Song Guan,Wen Bao Qi,Liang Quan Zhang,Ming Liao,Hai Tao Qi

doi:10.1186/1743-422x-8-469

Wei Li Kong, Pei Rong Jiao + Show 9 more

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https://doi.org/10.1186/1743-422x-8-469

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Journal: Virology Journal	Publication Date: Oct 13, 2011
Citations: 9	License type: cc-by

Affiliation: South China Agricultural University

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In China H3N2 and H1N1 swine influenza viruses have been circulating for many years. In January 2010, before swine were infected with foot and mouth disease in Guangdong, some pigs have shown flu-like symptoms: cough, sneeze, runny nose and fever. We collected the nasopharyngeal swab of all sick pigs as much as possible. One subtype H1N2 influenza viruses were isolated from the pig population. The complete genome of one isolate, designated A/swine/Guangdong/1/2010(H1N2), was sequenced and compared with sequences available in GenBank. The nucleotide sequences of all eight viral RNA segments were determined, and then phylogenetic analysis was performed using the neighbor-joining method. HA, NP, M and NS were shown to be closely to swine origin. PB2 and PA were close to avian origin, but NA and PB1were close to human origin. It is a result of a multiple reassortment event. In conclusion, our finding provides further evidence about the interspecies transmission of avian influenza viruses to pigs and emphasizes the importance of reinforcing swine influenza virus (SIV) surveillance, especially before the emergence of highly pathogenic FMDs in pigs in Guangdong.

Highlights

In China H3N2 and H1N1 swine influenza viruses have been circulating for many years
The first swine influenza virus (SIV) H1N2 was reported in Japan from 1978 to 1992 [8,9]
Phylogenetic analysis revealed that the HA gene of A/swine/Guangdong/1/2010 is of classical swine lineage resides in the same clade with pandemic (H1N1) 2009A/ California/04/2009, along with A/swine/Beijing/47/1991 (H1N1) and A/Swine/Indiana/9K035/99(H1N2) (Figure 2)

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Introduction

In China H3N2 and H1N1 swine influenza viruses have been circulating for many years. In January 2010, before swine were infected with foot and mouth disease in Guangdong, some pigs have shown flu-like symptoms: cough, sneeze, runny nose and fever. One influenza virus was isolated and named: A/swine/Guangdong/1/2010(H1N2). Phylogenetic analysis of A/swine/Guangdong/1/2010 (H1N2) was carried out by analyzing the data obtained here with those of other sequences of influenza viruses from GenBank database.

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