Abstract
Porcine epidemic diarrhea virus (PEDV), a leading cause of piglet diarrhea outbreaks, poses a significant danger to the swine industry. The aim of this study was to investigate the epidemic characteristics of PEDV that was circulating in Guangdong province, one of China’s major pig producing provinces. Clinical samples were collected from eight pig farms in Guangdong province between 2018 and 2019 and tested for the major porcine enteric pathogens, including PEDV, transmissible gastroenteritis virus (TGEV), Swine enteric coronavirus (SeCoV), Swine acute diarrhea syndrome coronavirus (SADS-CoV), porcine deltacoronavirus (PDCoV), and porcine rotavirus (RV). As a result, only PEDV and RV were detected at a rate of 47.0% (16/34) and 18.6% (8/34), respectively. Coinfectoin with PEDV and RV occurred at a rate of PEDV 12.5% (2/16). Subsequently, the full-length S gene sequences of 13 PEDV strains were obtained, and phylogenetic analysis suggested the presence of GII-c group PEDV strains in this region (non-S-INDEL). Two novel common amino acid insertions (55T/IG56 and 551L) and one novel glycosylation site (1199G+) were detected when the CV777 and ZJ08 vaccine strains were compared. Furthermore, intragroup recombination events in the S gene regions 51–548 and 2478–4208 were observed in the PEDV strains studied. In summary, the observations provide current information on the incidence of viral agents causing swine diarrhea in southern China and detailed the genetic characteristics and evolutionary history of the dominant PEDV field strains. Our findings will aid in the development of an updated vaccine for the prevention and control of PEDV variant strains.
Highlights
Several coronaviruses, including Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), SADS-CoV, Swine enteric coronavirus (SeCoV), and porcine deltacoronavirus (PDCoV), have been identified in piglets suffering from acute diarrhea and vomiting [29,30,31,32]
The molecular characterization of PEDVs is a major focus of swine diarrhea virus research due to the virus’s high prevalence
During 2018–2019, we investigated the presence of swine diarrhea virus in eight pig farms in Guangdong, China, and focused on the genetic characterization of PEDV
Summary
The aim of this study was to investigate the epidemic characteristics of PEDV that was circulating in Guangdong province, one of China’s major pig producing provinces. Several studies on the genetic characterization and prevalence of PEDV have been conducted in central [24] and western China [25] prior to 2018, there has been little research on the prevalence of PEDV in southern China in recent years. The purpose of this study was to determine
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