Abstract
The random amplified polymorphic DNA (RAPD-PCR) technique was examined as a potential tool for identification of genetic stock, gene diversity/gene frequencies studies. During this investigation, total 17 specimens from three study sites i.e., Upper Lake (Bhopal), Kshipra dam (Ujjain) and Wainganga river (Balaghat) were studied and delineated genetic studies and allele frequencies studies among the three populations. Total numbers of multiple loci/DNA fingerprints were 27 in all three populations of which number of polymorphic loci were in 8 in Upper Lake, 11 Kshipra Dam, 8 Wainganga River. Genetic differentiation (GST) among the populations was found to be as 0.5926, estimated gene flow between population as Nm=0.3437, intra-population heterozygosity as HS=0.1518 and total heterozygosity as HT=0.3726 clearly reflecting high genetic polymorphism. Nei’s unbiased genetic identity and genetic distance was obtained as highest distance between Upper Lake and Kishpra dam as 0.7223 however, less genetic distance was obtained between Wainganga River and Khipra dam populations. However, population wise, the genetic analyses in Upper Lake Bhopal indicated lower genetic polymorphism (P) as 29.63% as compared to rest of population’s i.e., Kshipra Dam 40.74% and Wainganga River 62.96%. Nei’s gene diversity (h) was observed as 0.1306 in Upper Lake Bhopal, 0.1288 in Kshipra Dam and 0.1956 Wainganga River reflecting much higher gene diversity in Wainganga River. This may be due to declined of genetic variation in the stocks which could cause by manipulation of habitat as the natural habitat or pressure of anthropogenic activities around the reservoir which may cause the threats of fish habitats.
Published Version
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