Genetic biodiversity of spiny lobsters (Panulirus spp.) from coastal waters of Southern Java, Indonesia

Abstract

Abstract. Haryono FED, Ambariyanto. 2018. Genetic biodiversity of spiny lobsters (Panulirus spp.) from coastal waters of Southern Java, Indonesia. Ocean Life 2: 73-78. Spiny lobsters of the genus Panulirus inhabit the southern Java, however, species identification of early life-stages based on morphological features analysis is equivocal. The condition as result of differences in morphology of the larval stage and the adult, a long period of larval life cycle, impact of oceanographic currents inhibit and restrict the route and direction of larval dispersal that causes genetic changes. However, genetic biodiversity of spiny lobsters from this area has not been investigated. The aim of this/our study was to identified genetic diversity of lobsters in Southern Java waters. The research was conducted from January through to August 2015. A total of 1137 lobsters were collected from seven different locations for morphological analyses and 40 lobsters were taken randomly for genetic analysis. Observations nuclear and mitochondrial DNA, and the relationships of genetic in phylogenetic using Codon of code and MEGA 5.0, and dendogram using Primer_e software. Morphological analysis obtained 6 species of spiny lobsters: P. homarus, P. versicolor, P. ornatus, P. penicillatus, P. polyphagus and P. longipes. Phylogenetic analysis obtained two clades and morphological identification results obtained P. penicillatus (spiny rock lobster), but the identification genetically acquired included in a group of genetic P. homarus (spiny sand lobster). Such discrepancies are possibly due to a long period of larval life, adult in low light of intensity environment and an extreme of oceanographic conditions with marine oil spill.